agar plates not solidifying food

Question about Agar Plates : biology

Question about Agar Plates discussion Hello I have been recently attempting to make agar plates to grow a slime mold but for some reason they have not been solidifying after 3 hours (as I plan to deprive them of food for the experiment) 15 comments share save hide report

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What is the difference between broth and agar media

The only difference between broth and agar media is that broths do not contain an agar component We use broth tubes primarily for specific assays or (rarely) for bacteria that will not form colonies on a solid surface Unlike preparation of agar plates tubes are prepared with media already in

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Potato Dextrose Agar (PDA)

1/12/2020The solidifying agent is agar To inhibit the growth of bacteria the pH level of the medium is lowered using a specific amount of 10% sterile tartaric acid To inhibit the overgrowth of competing microorganisms from the mixed specimen a selective agent is used in the form of chloramphenicol

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Mueller Hinton Agar (MHA)

10/26/2018Final pH 7 3 0 1 at 25C Principle of MHA Mueller Hinton Media contains Beef Extract Acid Hydrolysate of Casein Starch and Agar Beef Extract and Acid Hydrolysate of Casein provide nitrogen vitamins carbon amino acids sulphur and other essential nutrients Starch is added to absorb any toxic metabolites produced Starch hydrolysis yields dextrose which serves as a source of energy

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Blood Agar Base (Infusion Agar)

equilibrium and agar is the solidifying agent Supplementation with blood (5-10%) provides additional growth factors for fastidious microorganisms and is the basis for determining hemolytic reactions Hemolytic patterns may vary with the source of animal blood or type of base medium used 5 Formula BBL™ Blood Agar Base (Infusion Agar)

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Preparation of nutrient agar MicroDok microbiology

The preparation of nutrient agar is simple and the steps outlined in this section will only serve as a guide for you This image shows how nutrient agar looks like after preparation Components of nutrient agar base The components of nutrient agar base required for nutrient agar preparation include: Agar – which is the solidifying agent

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Agar Agar Gum

Agar is more appropriate for incubation purposes than other solidifying agents (e g gelatine) which melt near to human body temperature (37 C) In the Health industry Agar is used as an appetite suppressant and laxative since it comprises approximately 80% fibre and swells to three times its size when it absorbs water

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Nutrient Agar vs Blood Agar

Blood agar is almost identical to nutrient agar except that it contains five to ten percent sheep rabbit or horse blood Blood agar consists of: Beef extract for nitrogen Blood for nitrogen amino acids and carbon Sodium chloride for maintaining osmotic balance Agar for the solidifying agent Microbiologists use blood agar to identify

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Potato Dextrose Agar (PDA): principle composition and

Potato dextrose agar (PDA) contains dextrose as a carbohydrate source which serves as a growth stimulant and potato infusion that provides a nutrient base for luxuriant growth of most fungi Agar is added as the solidifying agent A specified amount of sterile tartaric acid (10%) may be incorporated to lower the pH of the medium to 3 5 so that

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Tips for Pouring and Storing Agar Plates

• Agar plates that contain nutrients (nutrient agar starch agar milk agar egg yolk agar L agar minimal agar with a sugar) can generally be used as long as they are not contaminated or dried out Examine the plates carefully for tiny microbial colonies that may have grown while the plates were stored

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Agar Powder Preparation Recipe

Agar is pronounced awger (sounds like fogger without the f) Agar is a gelling agent extracted from red seaweed Nutrient agar is a commonly used food medium for microbial cultures Nutrient agar contains: beef extract (provides carbohydrates nitrogen vitamins salts) peptone (helps control pH) agar (a carbohydrate used as a solidifying agent)

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Learn What Agar

Agar is often used as a laxative since it contains 80 percent fiber and moves through your digestive system fast This plant-based food doesn't have a true taste or smell but firms up in recipes unlike animal gelatin which has more of a creamy consistency In eastern traditional medicine agar has been used to treat illnesses like diabetes

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Learn What Agar

Agar is often used as a laxative since it contains 80 percent fiber and moves through your digestive system fast This plant-based food doesn't have a true taste or smell but firms up in recipes unlike animal gelatin which has more of a creamy consistency In eastern traditional medicine agar has been used to treat illnesses like diabetes

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How To Make Tomato Cages From Fencing : Suspectdh

Sterile agar plates can be pre-poured and stored in well-sealed plastic bags plate This involves the progressive dilution of an inoculum of bacteria or yeast agar plates not solidifying To make agar plates you use Petri dishes Petri is actually a person! A long time ago mister Petri was investigating bacteria using gelatine

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Salmonella Shigella agar preparation MicroDok

Salmonella-Shigella agar (SSA) is used for the selective cultivation and isolation of strains of Shigella species and Salmonella from clinically important samples It is both a selective and differential media and it can also be used to recover these organisms from environmental or non-clinical samples such as contaminated food samples

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