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Calculate the percentage of crossovers by dividing the number of crossover asci (2:2:2:2 or 2:4:2) by the total number of asci X 100 To calculate map distance divide the percentage of crossover asci by 2 The percentage of crossover asci is divided by 2 because only half the spores in each ascus are the result of a crossover event (Figure 3 3

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